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Oligo Analysis Tool

Our handy Oligo Analysis Tool calculates molecular weight (MW), extinction coefficient (E260), pmoles/µg, pmoles/OD and µg/OD. Also displayed for your convenience are the % GC Content, melting temp (TM), and the reverse complement of the oligo sequence you enter.


Oligos Calculations

The oligo sequence is displayed in the Oligo Analysis Plot below where the full-length of the primer is compared against itself for self-hybridization potential. To plot your oligo against other sequences or against shorter versions of itself, please enter a second sequence into the Plot Sequence box and click the CALCULATE button again. Areas of hybridization are marked with an X. Areas of 4 or more contiguous Xs are highlighted in yellow.

If the sequence is suitable for your experiment, you can use the ADD OLIGO TO CART button to order directly from this window.



* Sequence Name:
* Sequence: (5' to 3')
Sequence Length:
 

* Length indicates standard bases only
Scale:
Purification:

The graph below plots your sequence against itself. Complementary bases are marked, and primer-dimer sets are marked in bold. To plot your Oligo against a second sequence, please enter the sequence here:

Plot Sequence: (5' to 3')







Please enter your sequence information above and click the "CALCULATE" button.

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Eurofins MWG Operon, Inc. A Eurofins Genomics company. All rights reserved.
Eurofins Genomics | 2211 Seminole Drive | Huntsville, Alabama 35805, USA
E-mail questions/orders: oligo-us@eurofins.com | Web Comments: Operon_WebMaster@eurofins.com | Phone: (800) 688-2248