Important Note: Our laboratory will be closed Nov. 27th and Nov. 28th in observance of the holiday season. Please consider this change when placing orders.

Skip Navigation Links
Country Flag
United States
Skip Navigation Links Eurofins MWG Operon > Tools

Oligo Analysis Tool

Our handy Oligo Analysis Tool calculates molecular weight (MW), extinction coefficient (E260), pmoles/µg, pmoles/OD and µg/OD. Also displayed for your convenience are the % GC Content, melting temp (TM), and the reverse complement of the oligo sequence you enter.

Oligos Calculations

The oligo sequence is displayed in the Oligo Analysis Plot below where the full-length of the primer is compared against itself for self-hybridization potential. To plot your oligo against other sequences or against shorter versions of itself, please enter a second sequence into the Plot Sequence box and click the CALCULATE button again. Areas of hybridization are marked with an X. Areas of 4 or more contiguous Xs are highlighted in yellow.

If the sequence is suitable for your experiment, you can use the ADD OLIGO TO CART button to order directly from this window.

* Sequence Name:
* Sequence: (5' to 3')
Sequence Length:

* Length indicates standard bases only

The graph below plots your sequence against itself. Complementary bases are marked, and primer-dimer sets are marked in bold. To plot your Oligo against a second sequence, please enter the sequence here:

Plot Sequence: (5' to 3')

Please enter your sequence information above and click the "CALCULATE" button.

© 2014 
Eurofins MWG Operon, Inc. A Eurofins Genomics company. All rights reserved.
Eurofins Genomics | 2211 Seminole Drive | Huntsville, Alabama 35805, USA
E-mail questions/orders: | Web Comments: | Phone: (800) 688-2248