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Ultra Deep Sequencing of Exons and Genome Fragments

Sequencing of PCR amplicons with the Genome Sequencer™ FLX

New methods provide Ultra Deep Sequencing for specific applications, including the detection of rare mutations and the study of methylation patterns.

Our flexible services include consultation with our scientists to assist you with the design of the specific amplicons or design can be performed at our facilities as part of your project.

Specifications

• Delivery of amplicons with required adaptor sequences
• Pooling of samples is possible with specific sequence tags (barcodes)
• Generation of a GS shotgun library (optional) and emPCR
• Ultra deep sequencing with the GS FLX Titanium reagents
• Sorting of the sequences (and assembly, if required)

Your Advantages

• Support in experimental design
• Detection of rare somatic mutations
• Detection of rare SNPs in candidate populations
• Highest sensitivity for methylation studies