Transcriptome Sequencing Services – Proprietary Libraries Provide High Quality Data
Do you need a complete picture of the transcriptome of your organism of interest
or do you want to compare different expression levels in different samples?
For each of your requirements we are able to generate the appropriate library type
for sequencing.
Whole Transcriptome Sequencing with Normalized, Random-Primed cDNA Libraries
Get an excellent picture of whole transcripts by sequencing with our random-primed
cDNA libraries. To sequence the transcriptome as comprehensively as possible, the
libraries are normalized to equalize transcripts of varying amounts. With this approach,
sequencing can cover whole transcriptomes and even detect rare transcripts.
Our random-primed cDNA library is adapted to be sequenced on Roche GS FLX. Using
the long read lengths of 350-450 bp, most full size transcripts can be easily assembled.
Your advantages:
- Coverage of the whole transcriptome
- Detection of rare or yet unknown transcripts
- Finding new RNA viruses or anti-sense RNA (for poly(A)-tailed RNA)
Quantitative Expression Profiling with Proprietary 3’-Fragment cDNA Libraries
Excellent solutions to compare expression levels of transcripts from different samples
are available with our proprietary 3’-fragment cDNA libraries. These libraries allow
directional sequencing—from the end of the coding sequence into the 3’-UTR. Therefore,
reads can be easily anchored to a specific position of a reference sequence. Expression
levels are then determined by simply counting reads in different samples.
Our 3’-fragment cDNA libraries can be sequenced either on Roche GS FLX (long reads)
or on Illumina HiSeq 2000 (short reads). Sequencing on HiSeq 2000 guarantees quantitative
ultra deep sequencing and expression profiling.
Your advantages:
- Excellent library generation for limited amount of RNA (ng level)
- Comprehensive information for micro-array design and validation
- Possible annotation of reads (GS FLX only)
- Longer reads than with DGEP, SAGE, or super-SAGE provides non-ambiguous mapping
to the reference
- No restriction enzymes needed; no bias in library preparation