Whole Genome Sequencing Services by the Experts
Whole genome sequencing of pro- and eukaryotic genomes of any size—from shotgun coverage
sequencing to complete finishing and data analysis, including gap closure and editing.
de novo Sequencing Projects with State-of-the-Art Technology
High quality genome assemblies are obtained by sequencing a combination of shotgun
libraries and long paired-end (LPE) libraries on the Roche GS FLX.
The LPE fragments comprise two DNA sequence runs bridged by 3 kb, 8 kb, 20 kb, or
40 kb genome regions (jumping distance). This unique 4 library approach results
in the longest average scaffold sizes and smallest number of remaining gaps.
Proven experience in prokaryotic and eukaryotic high quality de novo genome sequencing.
In close cooperation with our customers, we perform the following de novo
genome sequencing projects:
- Ultra high throughput sequencing of a shotgun library and assembly into contigs
- Scaffolding of contigs by long paired-end sequencing
- Closing of gaps based on Sanger technology
- Bioinformatic analysis, e.g. annotation or comparison of different organisms
Resequencing Projects with the Technology of Choice
Depending on the aim of your studies, resequencing projects can be addressed using
either Illumina HiSeq 2000 or Roche GS FLX technology:
- Affordable resequencing with Illumina HiSeq 2000
For SNP detection and comparative analysis to a reference genome, we recommend the
cost-efficient resequencing setup with Illumina HiSeq 2000 technology.
- Sophisticated projects with Roche GS FLX
If large indels or genomic correlations between the sequenced and the reference
genome are likely to be detected, sequencing on Roche GS FLX is the best choice.
The longer reads of Roche GS FLX facilitate de novo assembly and subsequent comparative
analysis. With this approach, you benefit from high quality genome sequences, even
if large indels or genomic rearrangements occur.
Smaller-Sized Projects with Fast Turnaround Times with Roche GS Junior
The Roche GS Junior is the optimum system for the sequencing of smaller scale projects
(e.g. BACs) that require only one-eighth of the capacity of a GS FLX Titanium run
or would not be suitable for an Illumina run. As a consequence, the GS Junior adds
a higher degree of flexibility in project planning and can have a positive impact
on the turnaround time for small-sized projects.