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Re-Sequencing of Exons or Genome Fragments

Re-Sequencing of previously sequenced selected genomic regions (such as exons) to investigate correlations among sequence deviations and phenotypes.

Eurofins MWG Operon offers full services in 3 distinct phases from (I) primer design, primer synthesis and establishment of PCR conditions, (II) to high-throughput PCR and double strand sequencing , and (III) providing bioinformatics analyses to determine differences among references and samples.

Specifications

Phase I: Establishing PCR Strategies

• Primer design and synthesis for all exons regions
• Establishment of PCR amplification conditions for at least two DNA samples
• Double strand sequencing of the PCR fragments
• Two repetitions of PCR setup in the event of failure

Phase II: PCR and Sequencing

• High throughput PCR of all samples
• Purification of DNA from the PCR products
• High throughput sequencing of PCR DNA
• Single repetition in the event of sequencing failure

Phase III: Bioinformatic Analysis

• Double stranded data provide strongest support for highest quality results
• Identification of all differences among references and samples
• Detailed identification of homozygous and/or heterozygous substitutions
• Detection of insertions/deletions
• Mutation report and comparison of all samples provided

Your Advantages

• Reliable analysis of genetic variations
• High quality results and concise analysis format
• Fast turnaround times
• Consultancy and project support according to your experimental needs