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Amplicon Sequencing Services

Amplicon sequencing is based on ultra deep sequencing of PCR products for analyzing genetic variations. The long read lengths and data accuracy of Roche GS FLX makes the technology the gold standard for amplicon sequencing. As each amplicon molecule within a mixture of amplicons can be sequenced individually, the technology has the power to detect rare variants with detection limits of 0.5 % and even lower.

Amplicon Variance Analysis Supported by Dedicated Bioinformatic Solutions

Amplicon variance analysis is ideally suited for highly variant samples, such as the hypervariable regions of antibodies, and samples with low variance, such as population samples for SNP detection.

Typical projects are:

• Diversity screenings within populations of individuals
• Identification of rare mutations associated with diseases (SNP detection)
• Metagenome analysis (e.g. 16S or 18S rDNA analysis)
• Study of methylation patterns

For amplicon sequencing, PCR products have to be linked with certain adaptors to generate appropriate libraries for next generation sequencing.

Amplifying your sequences of interest with fusion primers is the method of choice.

Fusion primers consist of a target-specific sequence at the 3' end and a fixed sequence at the 5’end (Primer A and Primer B). For sequencing several samples in parallel, several barcoded samples may be pooled. Barcoding of amplicon samples is done by insertion of multiplex identifier tags (MIDs) into the fusion primers (see figure).