Primer Walking DNA Sequencing Services

Eurofins MWG Operon offers Primer Walking DNA sequencing service with the best possible delivery times by making use of our in-house custom DNA synthesis capabilities. No other DNA sequencing provider has the experience and capabilities in custom primer design and synthesis that Eurofins MWG Operon has.

Single-Strand DNA Sequencing

If you need the complete DNA sequence of a plasmid insert or PCR fragment which is longer than the average read length of a single DNA sequencing run (750-900 bases), we recommend ordering the single-strand Primer Walking DNA sequencing service.

Double-Strand DNA Sequencing

De novo DNA sequencing typically requires sequencing of both strands of the template to establish and confirm each base. Sequencing both strands of a sample results in a higher accuracy of the DNA sequencing data and provides confirmation of the reads. We begin by sequencing into the insert using primers specific to each strand. We then extend the PHRED 30 information using newly designed and synthesized primers derived from the results of the prior run. This process is continued until the entire template is sequenced on both strands. If there are any ambiguities in the DNA sequence, we will design further primers and run additional reactions to resolve the ambiguous calls. This process ensures publication-grade DNA sequencing results with a minimum accuracy of 99.995% for your insert.

Order Primer Walking DNA Sequencing Services via E-mail by sending a completed order form (download below) to seqsupport@eurofins.com