Stock Oligos
Overview
Eurofins MWG Operon supplies a large number of universal sequencing primers as well as stock oligos. High-quality primers and oligos synthesized under salt-free conditions using proprietary technologies. Stock primers and oligos are synthesized with high coupling efficiency under salt- free conditions using proprietary synthesis technologies, and show excellent performance. All sequencing primers are HPLC purified. Other stock oligos are salt-free.
Specifications
Stock oligos and primers are provided lyophilized in 2 ml tubes.
Purity Level: Salt-Free or HPLC as stated on the ordering page.
Yield:
- As stated in ordering page
- 1 OD ≈ 33 µg ssDNA
Storage and Handling
Unmodified oligos can be used for at least 12 months after purchase when stored wet at -20°C. For long-term storage, oligos should be stored dry at -20°C. If numerous experiments are planned using the same sequence, aliquot the sample, dry all aliquots, and store at -20°C. If the oligos are stored wet, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the oligo. Oligos generally last longer in TE than in water. Careful handling is recommended to avoid the possibility contamination with nucleases or bacteria.
Before opening, spin the tube at 1000 rpm for 5 min to ensure that the oligonucleotides are at the bottom of the tube. Oligonucleotides should be resuspended in a sterile buffered solution (e.g., TE at pH 7.0). Vortex oligonucleotides thoroughly after resuspension. The oligonucleotides may not readily dissolve in sterile, distilled water. The addition of NaOH to the water until the pH rises to 7.0 may help. If the oligonucleotides are resuspended at pH <7.0 (deionized water may have a pH as low as 5.0), the oligonucleotide could begin to degrade and may lose functionality within a couple of weeks.
For optimal long-term storage, oligonucleotides should be stored dry at -20°C in the dark. If numerous experiments are planned using the same oligonucleotide, prepare aliquots, dry them and store the aliquots at -20°C. Use clean, sterile labware for all transfers.