DNA Sequencing Services
With over a decade of experience and thousands of successful projects, Eurofins MWG Operon proudly offers DNA sequencing services that you can rely on. We use the proven technology of Geospiza GeneSifter® Lab Edition software to enable automated tracking of sample barcodes and secure access to your results through our web interface. And, to manage your DNA sequencing and synthesis purchases, we offer prepayment options using the EVOcard.
Service Overview
Automated DNA Sequencing Services
Our automated DNA sequencing services yield fast and reliable results for PCR products, plasmid inserts, and large clones. Samples can be submitted in tubes or 96-well plates. Use of our bar-coded labels ensures a unique identifier for each template and primer submitted, allowing quick, accurate, and secure tracking and viewing of your results. Results are typically available within 24 hours from the receipt of your samples.
Barcodes for Unique ID
Bar-coded labels for templates and enclosed primers provide a unique identifier for your DNA sequencing results. These simple-to-use labels are available free of charge from our website. Click here to order yours.
Power Read DNA Sequencing Service
Some templates need a little something extra to ensure proper results. Our Power Read DNA sequencing service is customized to power through any expected—and unexpected—difficulties with your template. It includes individual support at every stage and optimized DNA sequencing chemistry. If the first read fails, another read will be performed using adjusted chemistry. Suitable for a variety of samples, the Power Read DNA sequencing service is particularly recommended for templates with high GC or GT content, secondary structure, or long repeats.
Large Clone DNA Sequencing Service
Eurofins MWG Operon offers a DNA sequencing service for inserts in large cloning vectors. Vectors larger than 20 kBp (e.g. BACs, PACs, cosmids, fosmids) are read using specialized DNA sequencing chemistry that is proven to optimize results for these samples.
Ready to Load DNA Sequencing Services
You provide the pre-cycled DNA sequencing reactions in tubes or 96-well plates and we provide the rest. Eurofins MWG Operon runs your reactions on our ABI 3730xl DNA Analyzers, and provides a quality report and the DNA sequence results.
Primer Walking DNA Sequencing Service
For DNA sequence analysis of a template that is longer than the average read length (750-900 bases) of a single DNA sequencing run, or for publication grade results (min. accuracy of 99.995%) we offer sequencing of one or both strands of your insert.
All of our Single Read Sequencing Services include:
- Personal order preferences and easy order tracking
- Read lengths up to 1100 Q20 bases*
- Quality report along with your sequence data
- Confidential services and secure download of data
*Q20: quality bases with a minimal score of 20 as calculated by the ABI KB™ basecaller (comparable with PHRED 20 and roughly represents 1 in 100 bases called in error)
Automated DNA Sequencing Services
Let Eurofins MWG Operon take over your daily DNA sequencing work. Providing a fast and convenient way to sequence plasmids, PCR products, and large clone inserts, our automated DNA sequencing services are your best choice for fast and reliable DNA sequencing. Our automated services allow rapid processing of for all template types either in tubes or 96-well plates.
Specifications
- 24-hour service (after receiving sample)
- Available for tubes and 96-well plates
- Plates must contain a minimum of 48 reactions
- Bar-coded labels available for unique identification of templates and primers
- Specific primers can be requested or enclosed
- Free standard primers available
- Template and primer may be sent premixed
- Accuracy of data is greater than 99% (Q20 or better)
- Prepayment option with the EVOcard
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Barcodes for Unique ID
Eurofins MWG Operon provides bar-code labels for use with templates and primers that are submitted for DNA sequencing services. These labels can be ordered free of charge by clicking here. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins MWG Operon for DNA sequencing has a unique identifier that is tracked throughout our automated system. These barcodes are also prominently displayed on your results retrieval page to give you added assurance that your DNA sequencing results correlate to the samples submitted.
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Power Read DNA Sequencing Service
Templates with high GC or GT content, secondary structure, long repeats, or other difficult to sequence areas often need specialized reaction conditions to ensure quality results. The Power Read DNA sequencing service is particularly recommended for these difficult templates.
Don’t just re-read; Power Read!
Important: The Power Read service is not just a promise to run the reaction again if it fails the first time. In our experience, if a reaction doesn’t work the first time, it won’t work the second time, either. The Power Read service includes an optimization of template concentration, as well as reaction chemistry, to maximize the success of the first DNA sequencing reaction. If, for any reason, the first read was unsuccessful, we will analyze the results and adjust the concentration and/or chemistry before running the second DNA sequencing reaction. This truly increases the chances for a successful read, even with the most difficult to sequence templates.
Additional Benefits of the Power Read Service
- Individualized scientific consultation for your specific DNA sequencing needs
- Quantitation and adjustment of DNA template to optimize concentration and volume
- Optimization of DNA sequencing reaction chemistry for your primer and template
- A second DNA sequencing reaction, if necessary, under adjusted conditions
- Excess template storage for at least 30 days
- Personal review of DNA sequencing results
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Large Clone DNA Sequencing Service
Eurofins MWG Operon provides automated DNA sequencing of larger insert clones (e.g. BACs, PACs, cosmids, fosmids) using Sanger DNA sequencing technology to support:
- Creation of physical maps (minimal tiling path)
- Scaffolding of contigs
- Gap closure through end sequencing and/or PCR methods
- Coverage sequencing of defined BAC clones
Physical Gap Closure of Larger Insert Clones
- Scaffolding and final gap closure by various PCR approaches
- Primer walking steps on large insert DNA templates
- Additional bioinformatics services on request
Shotgun Sequencing of Larger Insert Clones using Sanger Technology
- Clones can be delivered as stab or glycerol cultures and we’ll prepare the DNA
- Generation of shotgun plasmid libraries
- DNA sequencing until requested coverage is achieved
- Assembly of the sequence reads
Additional Benefits of the Large Clone DNA Sequencing Service
- Fast turnaround times
- Project consultancy and support
- Storage of material and data
- Highly experienced bioinformatics services
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Ready to Load DNA Sequencing Services
If you prefer to run your own BigDye® reactions but need someone to read your samples and provide the final DNA sequencing results, Eurofins MWG Operon can help. We will perform the dye terminator removal, run your reaction on our ABI 3730xl DNA sequencers, and provide a quality report and the DNA sequence results.
Specifications
- 24-hour service
- Available for tubes and 96-well platest
- Dye terminator removal performed in our lab
- DNA sequencing read using standard parameters on ABI 3730xl DNA sequencers
- Prepayment option with the EVOcard
The quality of data for the Ready to Load DNA sequencing service is not guaranteed by Eurofins MWG Operon, as critical factors in DNA sequencing, including DNA preparation and reaction conditions, are not performed in our labs. For many labs, however, our Ready to Load DNA sequencing service provides the best value for obtaining reliable DNA sequence data.
Order Now – Ready to Load DNA Sequencing Services
Primer Walking DNA Sequencing Services
Eurofins MWG Operon offers Primer Walking DNA sequencing service with the best possible delivery times by making use of our in-house custom DNA synthesis capabilities. No other DNA sequencing provider has the experience and capabilities in custom primer design and synthesis that Eurofins MWG Operon has.
Single-Strand DNA Sequencing
If you need the complete DNA sequence of a plasmid insert or PCR fragment which is longer than the average read length of a single DNA sequencing run (750-900 bases), we recommend ordering the single-strand Primer Walking DNA sequencing service.
Double-Strand DNA Sequencing
De novo DNA sequencing typically requires sequencing of both strands of the template to establish and confirm each base. Sequencing both strands of a sample results in a higher accuracy of the DNA sequencing data and provides confirmation of the reads. We begin by sequencing into the insert using primers specific to each strand. We then extend the PHRED 30 information using newly designed and synthesized primers derived from the results of the prior run. This process is continued until the entire template is sequenced on both strands. If there are any ambiguities in the DNA sequence, we will design further primers and run additional reactions to resolve the ambiguous calls. This process ensures publication-grade DNA sequencing results with a minimum accuracy of 99.995% for your insert.
Order Primer Walking DNA Sequencing Services via E-mail by sending this completed order form (download below) to seqsupport@mwgdna.com
Order Form: Excel 97, 98, 2000, XP, 2003 (.xls)
Sample Submission Guidelines