DNA Sequencing Services
With over a decade of experience and thousands of successful projects, Eurofins
MWG Operon proudly offers DNA sequencing services that you can rely on. We use the
proven technology of Geospiza GeneSifter® Lab Edition software to enable automated
tracking of sample barcodes and secure access to your results through our web interface.
And, to manage your DNA sequencing and synthesis purchases, we offer prepayment
options using the EVOcard.
Service Overview
Automated DNA Sequencing Services
Our automated DNA sequencing services yield fast and reliable results for PCR products,
plasmid inserts, and large clones. Samples can be submitted in tubes or 96-well
plates. Use of our bar-coded labels ensures a unique identifier for each template
and primer submitted, allowing quick, accurate, and secure tracking and viewing
of your results. Results are typically available within 24 hours from the receipt
of your samples.
Barcodes for Unique ID
Bar-coded labels for templates and enclosed primers provide a unique identifier
for your DNA sequencing results. These simple-to-use labels are available free of
charge from our website. Click here to
order yours.
Power Read DNA Sequencing Service
Some templates need a little something extra to ensure proper results. Our Power
Read DNA sequencing service is customized to power through any expected—and unexpected—difficulties
with your template. It includes individual support at every stage and optimized
DNA sequencing chemistry. If the first read fails, another read will be performed
using adjusted chemistry. Suitable for a variety of samples, the Power Read DNA
sequencing service is particularly recommended for templates with high GC or GT
content, secondary structure, or long repeats.
BAC DNA Sequencing Service
Eurofins MWG Operon offers a DNA sequencing service for BAC inserts, as well as
inserts in other large cloning vectors (e.g. PACs, cosmids, fosmids). BAC inserts
are read using specialized DNA sequencing chemistry that is proven to optimize results
for these samples. We also offer BAC End Sequencing Service that includes bacterial
culturing, DNA extraction and prep, and sequencing of the insert from both ends.
Express Plate™ DNA Sequencing Service
Express results for DNA sequencing reactions! Submit your samples with template
and primer premixed in a 96-well plate and, once we receive your samples, we'll
send results to you—typically 550 bases—by 9 am the next morning.
Primer Walking DNA Sequencing Service
For DNA sequence analysis of a template that is longer than the average read length
(750-900 bases) of a single DNA sequencing run, or for publication grade results
(min. accuracy of 99.995%) we offer sequencing of one or both strands of your insert.
All of our Single Read Sequencing Services include:
- Personal order preferences and easy order tracking
- Read lengths up to 1100 Q20 bases*
- Quality report along with your sequence data
- Confidential services and secure download of data
*Q20: quality bases with a minimal score of 20 as calculated by the ABI KB™ basecaller
(comparable with PHRED 20 and roughly represents 1 in 100 bases called in error)
Automated DNA Sequencing Services
Let Eurofins MWG Operon take over your daily DNA sequencing work. Providing a fast
and convenient way to sequence plasmids, PCR products, and large clone inserts,
our automated DNA sequencing services are your best choice for fast and reliable
DNA sequencing. Our automated services allow rapid processing of for all template
types either in tubes or 96-well plates.
Specifications
- 24-hour service (after receiving sample)
- Available for tubes and 96-well plates
- Plates must contain a minimum of 48 reactions
- Bar-coded labels available for unique identification of templates and primers
- Specific primers can be requested or enclosed
- Free standard primers available
- Template and primer may be sent premixed
- Accuracy of data is greater than 99% (Q20 or better)
- Prepayment option with the EVOcard
Order Now – Automated DNA Sequencing Services
Barcodes for Unique ID
Eurofins MWG Operon provides bar-code labels for use with templates and primers
that are submitted for DNA sequencing services. These labels can be ordered free
of charge by clicking here. By using bar-coded
labels, you guarantee that each sample and primer submitted to Eurofins MWG Operon
for DNA sequencing has a unique identifier that is tracked throughout our automated
system. These barcodes are also prominently displayed on your results retrieval
page to give you added assurance that your DNA sequencing results correlate to the
samples submitted.
Order Now – DNA Sequencing Bar-Coded
Labels
Power Read DNA Sequencing Service
Templates with high GC or GT content, secondary structure, long repeats, or other
difficult to sequence areas often need specialized reaction conditions to ensure
quality results. The Power Read DNA sequencing service is particularly recommended
for these difficult templates.
Don’t just re-read; Power Read!
Important: The Power Read service is not just a promise to run
the reaction again if it fails the first time. In our experience, if a reaction
doesn’t work the first time, it won’t work the second time, either. The Power Read
service includes an optimization of template concentration, as well as reaction
chemistry, to maximize the success of the first DNA sequencing reaction. If, for
any reason, the first read was unsuccessful, we will analyze the results and adjust
the concentration and/or chemistry before running the second DNA sequencing reaction.
This truly increases the chances for a successful read, even with the most difficult
to sequence templates.
Additional Benefits of the Power Read Service
- Individualized scientific consultation for your specific DNA sequencing needs
- Quantitation and adjustment of DNA template to optimize concentration and volume
- Optimization of DNA sequencing reaction chemistry for your primer and template
- A second DNA sequencing reaction, if necessary, under adjusted conditions
- Excess template storage for at least 30 days
- Personal review of DNA sequencing results
Order Now – Power Read DNA Sequencing Services
BAC DNA Sequencing Service
Eurofins MWG Operon provides automated DNA sequencing of BAC clones (as well as
other large clone inserts, e.g. PACs, cosmids, fosmids) using Sanger DNA sequencing
technology. We offer 2 services:
BAC Read DNA Sequencing Service
This service consists of a single pass read of your BAC clones using standard or
custom primers. Special chemistry and expert handling of the reactions enables typical
read lengths of over 750 bases. BAC Read DNA sequencing service can be used to sequence
inserts in many large clone vectors, including BACs, PACs, cosmids, and fosmids.
Order Now
– BAC Read DNA Sequencing Service
BAC End Sequencing Service
This full featured service includes bacterial culture, BAC clone prep, and sequencing
the insert from both ends. Bacteria can be delivered as stabs, glycerol stocks,
or colonies.
Additional Benefits of BAC End Sequencing Service
- Fast turnaround times
- Project consultancy and support
- Storage of material and data
Order BAC End Sequencing Services via E-mail by sending this completed order form
(download below) to seqsupport@operon.com
Order Form:
Excel 97, 98, 2000, XP, 2003 (.xls)
Express Plate™ DNA Sequencing Service
Submit your samples in a 96 well plate and your results will be ready by 9 am the
morning after we receive your samples. The express service has a typical read length
of 550 bases and is available for DNA Read, Power Read, and Ready to Load reactions.
Specifications
- Express results (by 9 am the morning after we receive your samples)
- Per plate pricing - up to 94 samples (up to 95 samples for Ready to Load samples)
- Typical read length of 550 bases
Ready to Load Samples
- Samples must be delivered post BigDye® reaction
- Dye terminator removal must be performed prior to sample submission
- DNA sequencing read using standard parameters on ABI 3730xl DNA sequencers
The quality of data for the Ready to Load DNA sequencing service is not guaranteed
by Eurofins MWG Operon, as critical factors in DNA sequencing, including DNA preparation
and reaction conditions, are not performed in our labs. For many labs, however,
our Ready to Load DNA sequencing service provides the best value for obtaining reliable
DNA sequence data.
Order Now – Express Plate DNA Sequencing Service
Primer Walking DNA Sequencing Services
Eurofins MWG Operon offers Primer Walking DNA sequencing service with the best possible
delivery times by making use of our in-house custom DNA synthesis capabilities.
No other DNA sequencing provider has the experience and capabilities in custom primer
design and synthesis that Eurofins MWG Operon has.
Single-Strand DNA Sequencing
If you need the complete DNA sequence of a plasmid insert or PCR fragment which
is longer than the average read length of a single DNA sequencing run (750-900 bases),
we recommend ordering the single-strand Primer Walking DNA sequencing service.
Double-Strand DNA Sequencing
De novo DNA sequencing typically requires sequencing of both strands of the template
to establish and confirm each base. Sequencing both strands of a sample results
in a higher accuracy of the DNA sequencing data and provides confirmation of the
reads. We begin by sequencing into the insert using primers specific to each strand.
We then extend the PHRED 30 information using newly designed and synthesized primers
derived from the results of the prior run. This process is continued until the entire
template is sequenced on both strands. If there are any ambiguities in the DNA sequence,
we will design further primers and run additional reactions to resolve the ambiguous
calls. This process ensures publication-grade DNA sequencing results with a minimum
accuracy of 99.995% for your insert.
Order Primer Walking DNA Sequencing Services via E-mail by sending this completed
order form (download below) to seqsupport@operon.com
Order Form:
Excel 97, 98, 2000, XP, 2003 (.xls)
Sample Submission Guidelines