Re-Sequencing of Exons or Genome Fragments
Re-Sequencing of previously sequenced selected genomic regions (such as exons) to
investigate correlations among sequence deviations and phenotypes.
Eurofins MWG Operon offers full services in 3 distinct phases from (I) primer design,
primer synthesis and establishment of PCR conditions, (II) to high-throughput PCR
and double strand sequencing , and (III) providing bioinformatics analyses to determine
differences among references and samples.
Specifications
Phase I: Establishing PCR Strategies
- Primer design and synthesis for all exons regions
- Establishment of PCR amplification conditions for at least two DNA samples
- Double strand sequencing of the PCR fragments
- Two repetitions of PCR setup in the event of failure
Phase II: PCR and Sequencing
- High throughput PCR of all samples
- Purification of DNA from the PCR products
- High throughput sequencing of PCR DNA
- Single repetition in the event of sequencing failure
Phase III: Bioinformatic Analysis
- Double stranded data provide strongest support for highest quality results
- Identification of all differences among references and samples
- Detailed identification of homozygous and/or heterozygous substitutions
- Detection of insertions/deletions
- Mutation report and comparison of all samples provided
Your Advantages
- Reliable analysis of genetic variations
- High quality results and concise analysis format
- Fast turnaround times
- Consultancy and project support according to your experimental needs