Gene Synthesis Services

Standard Genes

GC content from 40-65%
Homopolymer stretches less than 20 bp; repeats do not exceed 20 bp
No critical hairpin structures

Complex Genes

Long repeats and secondary structure may be present
Review of your sequence by our scientists may provide tips to increase your experimental success

Gene Evolution Libraries

Gene Evolution Libraries are ideally suited for protein mutation screening strategies often used by researchers to probe the potential properties of a protein. Our gene synthesis service scientists can place mutations in single or multiple positions within specific domains or through randomized base substitutions (incorporation of pre-selected base mixtures at designated positions). Gene Evolution Libraries can be delivered as linear DNA or as cloned libraries.

Our Gene Synthesis Process

1. Optimization (when requested)
   Review of your Gene Synthesis construct for potential repeat or hairpin structures, G/C content and base distribution, and strategies for modifying the construct when desired.
2. Codon Usage Optimization (when requested)
   Utilizing GENEius sequence optimizing software, the amino acid or DNA sequence you submit is adapted to the codon usage of the target organism.
3. Construct Design and Oligo Synthesis
   Overlapping oligonucleotides are designed to support the optimal gene construction method. These component oligos are then synthesized for high-performance characteristics in gene synthesis by our in-house production facility
4. Gene Synthesis
   Synthetic DNA oligonucleotides are assembled in order with subsequent strand closure employing LCR (Ligase Chain Reaction) and/or other PCR techniques.
5. Subcloning
   The completed gene is cloned into one or more standard or customer requested vectors.
6. DNA Sequence Verification of the Gene Construct
   The correct base sequence is confirmed through DNA sequencing of both strands at our in-house sequencing lab. Your gene construct is guaranteed by Eurofins MWG Operon as 100% correct.

Delivery and Documentation

Each synthetic DNA gene is delivered as plasmid DNA (mini scale preparation). Higher scales are available on request. Gene Evolution Libraries are delivered as linear DNA or fully cloned libraries. Our documentation of your construct includes a Quality Assurance Report which details the agreed project specifications, and includes a complete plasmid description, restriction map and the DNA sequence verification data for the synthetic gene.