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Gene Synthesis

Overview

Accelerate your research with our synthetic gene tools and services! Eurofins MWG Operon offers the highest quality synthetic genes. Our services include employing GENEius sequence optimization software for customizing codon usage and optimizing of your construct. Enjoy short turnaround times along with the highest quality in products and services.

De Novo Synthesis of Double Stranded DNA

Starting from DNA or amino acid sequence data in electronic format, Eurofins MWG Operon synthesizes your DNA fragment of interest with no length restrictions. Every synthetic gene is cloned into one of our standard vectors or into the vector of your choice. 100% accuracy of gene synthesis is guaranteed and verified through DNA sequencing.

• Eurofins MWG Operon produces synthetic genes useful for a large range of applications
• Protein translation optimization in heterologous expression systems through codon usage
  adapted
• Gene expression optimization
• Protein over expression
• Protein engineering services
• Real-Time PCR standards and controls
• Hybrid gene construction by rearrangement of domains and deletion or addition of
  introns
• Production of DNA vaccines
• Creation of multiple gene variants to screen for improved properties in proteins

Our Gene Synthesis Process

DNA Structure Optimization (when requested) – Review of your construct for potential repeat or hairpin structures, G/C content and base distribution, and strategies for modifying the construct.

Codon Usage Optimization (when requested) – Adaptation of the amino acid or DNA sequence to the codon usage of the target organism.

Construct Design and Oligo Synthesis – Design and in-house synthesis of overlapping oligonucleotides to support the optimal gene construction method.

Gene Assembly – Synthetic DNA oligonucleotides are assembled in order with subsequent strand closure employing LCR (Ligase Chain Reaction) and/or other PCR techniques.

Subcloning – The completed gene is cloned into one or more standard or customer requested vectors.

DNA Sequence Verification of the Gene Construct – The correct sequence is confirmed through DNA sequencing of both strands at our in-house sequencing lab. Your gene construct is guaranteed by Eurofins MWG Operon as 100% correct.

Gene Evolution Libraries

Gene Evolution Libraries are ideally suited for protein mutation screening Eurofins MWG Operon can place mutations in single or multiple positions within specific domains or through randomized base substitutions (incorporation of pre-selected base mixtures at designated positions). These Gene Evolution Libraries can be delivered as linear DNA or as cloned libraries.

Specifications

Standard Genes

• GC content from 40-65%
• Homopolymer stretches less than 20 bp; repeats do not exceed 20 bp
• No critical hairpin structures

Complex Genes

• GC content <40% or >65%
• Long homopolymer stretches, long repeats, and/or secondary structure may be present
• Review of your sequence by our scientists may provide tips to increase your experimental success

Delivery and Documentation

Each synthetic DNA gene is delivered as plasmid DNA (mini-scale preparation). Higher scales are available upon request. Gene Evolution Libraries are delivered as linear DNA or fully cloned libraries. Our documentation of your construct includes:

• Quality Assurance Report – details the agreed project specifications
• Complete plasmid description
• Restriction map
• DNA sequence verification data