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Standard Genes

Genes in 8 Days or it's free!

Your gene from 160 to 1000 bp is synthesized for 35¢/bp in 8 days or it's free!

Details of the 8-Day Guarantee

Eurofins MWG Operon offers standard genes which are available in any size, with moderate GC content (40-65%), without extensive repeats (> 20 bp) or critical hairpin structures and without extensive homopolymer stretches (> 20 bp). Our proprietary software GENEius provides unique features for gene optimisation.

Processing Standard Genes

1. Sequence Optimization (optional)

By using our proprietary software, GENEius, the supplied amino acid or DNA sequences will be optimized, using our proprietary 4D™ Optimization process. Codons are optimized for the host species, repeats or hairpin structures are avoided, and G's and C's are equally distributed. GENEius is also available as free online tool for our customers.

2. Experimental Design

Overlapping oligonucleotides used to create the gene are designed by GENEius.

3. Oligonucleotide Synthesis

All oligonucleotides are synthesized at our in-house DNA synthesis facility.

4. Gene Synthesis

Synthetic DNA is assembled using overlapping oligonucleotides, employing LCR (Ligase Chain Reaction) and PCR technologies.

5. Subcloning

The gene can be cloned into a standard vector or on request into a vector of your choice.

6. Quality Control

All genes undergo double-strand DNA sequencing at our in-house sequencing facility, to ensure that each gene has the proper sequence.

Delivery and Documentation

The synthetic DNA will be delivered as plasmid DNA (mini scale preparation). Higher scales are available on request. You receive a quality assurance report, including detailed project specifications, complete plasmid description, restriction map and DNA sequence information. The following special delivery options are available upon request: bacteria stabs, wet plasmid DNA, dry or wet linearized plasmid DNA, and PCR products.

We ensure strict confidentiality of every project. The high quality standard of our service is permanently assured by a professional quality management. The quality management system of our Huntsville, Alabama facility is certified to ISO 9001:2008 and ISO 13485:2003 quality standards.

© 2013 
Eurofins MWG Operon. All rights reserved.
Eurofins MWG Operon | 2211 Seminole Drive | Huntsville, Alabama 35805, USA
E-mail questions/orders: oligo-us@eurofins.com | Web Comments: Operon_WebMaster@eurofins.com | Phone: (800) 688-2248