Custom Oligo Modifications
Eurofins MWG Operon offers a wide variety of modifications. All modified oligonucleotides
are available at 200nmol and 1umol synthesis scales. Some are also available in
the 50nmol and 10umol scale. The maximum length of oligonucleotides with a 3' modification
is 40 bases. Independent of the application, some modifications need further purification.
Detailed information for each modification, a designator for ordering, and the chemical
structure can be found by following the hyperlink below the respective category
description.
Available modification categories:*
*Additional modifications are available. Please contact
us.
Information for modifications and corresponding applications and vice versa
can be found here.
Attachment Mods
Amino, thiol, acrydite, and phosphate groups incorporated during synthesis allow post synthesis reactions for attaching a variety of labels or for coupling the oligo with other molecules.
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Biotins
Biotin offers a variety of uses in research including assays in drug discovery, histological, and cytological applications and capture methods are based on the high affinity of biotin for avidin and streptavidin.
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DNA Analogs
Molecules with similar structure to DNA often readily substitute in the DNA strand for a variety of experimental uses. Modifications in this category include inosine, uridine, wobbles, etc.
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Dual-labeled Probes
A selected grouping of highly popular Fluorophore/Quencher pairings for real-time/qPCR applications.
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Fluorophores
Fluorescent molecules offer a wide variety of applications requiring detection of hybridization including in situ probes, sequencing, and genotyping.
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Miscellaneous
Miscellaneous modifications include phosphorothioate backbone modification, digoxigenin labeling, modified bases to prevent extension of the 3' terminus, cholesterol for cell delivery, puromycin (a tRNA mimc), etc.
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Mixed Bases
Standard bases used in combination to create a base comprised of a mixture of 2-4 bases (e.g. A/C or A/C/G). This category includes IUB Wobbles and custom wobbles.
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Modified Bases
Chemically modified bases can be used for structural studies and enhanced binding. This category includes Br-dU, 5-methyl dC, 5-iodo dU, 2-aminopurine, etc.
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Quenchers
Quenchers are most commonly paired with specific fluorophores and are chosen based on their ability to absorb energy at the emission wavelength of the fluorophore.
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RNA Analogs
RNA analogs are useful for creating the chimeric DNA/RNA oligos commonly used in anti-sense studies.
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Spacers
Spacers form a non-binding bridge between areas of an oligo. Among our offerings are C3 spacer which can substitute for an unknown base and Spacer 18 which introduces a hydrophobic area in an oligo.
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References
Tyagi S, Bratu D, Kramer FR, Multicolor molecular beacons for allele discrimination,
Nature Biotechnology 1998, 16: 49-53.
Kramer FR, Tyagi S, Vet JAM, Marras SAE, Molecular beacons: hybridization probes
for detection of nucleic acids in homogeneous solutions,
http://www.molecular-beacons.org.
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