Yield Specifications
To determine the scale for synthesis, the tables below detail the guaranteed minimum yield for various categories of oligo synthesis. Oligo synthesis often results in more material than listed here, however if more than the minimum yield will be required for your experiments then we highly recommend that a higher synthesis scale be ordered. More complex oligos which are not detailed below are highly dependent on a variety of oligos and/or modification properties and, for this reason, specific yields are not guaranteed.
Minimum yields are guaranteed for both standard and Express Oligos®. Yields are given in units of optical density (OD). For more information, see Calculations or contact our Technical Support team.
For additional information on which purity level is recommended for your specific application, please refer to our Oligo Applications Guide.
Salt Free
Salt-Free is our basic oligo purity option and is appropriate for most unmodified
oligos used in many applications, including routine PCR, microarray fabrication,
and blotting.
Guaranteed minimum yield for Salt-Free purity Standard and Express Oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | 0.5 | 0.5 | 1.0 | 4.0 | 14 | 90 |
| 10-14 | 1.0 | 2.0 | 3.0 | 7.0 | 24 | 160 |
| 15-19 | 2.0 | 3.0 | 4.0 | 11 | 36 | 240 |
| 20-45 | 2.0 | 3.0 | 6.0 | 15 | 48 | 320 |
| 46-60 | 2.0 | 3.0 | 12 | 35 | 100 | 740 |
| 61-69 | NA | NA | 12 | 45 | 148 | 980 |
| 70-80 | NA | NA | 14 | 45 | 148 | 980 |
| 81-90 | NA | NA | 14 | 29 | 148 | NA |
| 91-100 | NA | NA | NA | 29 | 148 | NA |
| 101-125 | NA | NA | NA | 29 | 148 | NA |
HPSF® Purity
High purity salt free (HPSF) purification is a high-performance, high-throughput
purification that efficiently removes most N-1 and other truncated sequences created
during oligo synthesis. When using unmodified oligos in more sensitive applications
such as qPCR or DNA sequencing, HPSF delivers excellent yields and improved purity.
HPSF is available and recommended for oligos containing:
- IUB Wobbles (degenerate nucleotide sites)
- 5' Amino C6 Linker [Amino C6]
- 5' Phosphate [Phos]
- 5' Biotin-TEG [BioTEG] *
* 90 bases maximum length
Guaranteed minimum yields
(OD) for HPSF purity standard and modified oligos (as listed above)
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | 0.5 | 0.5 | 1.5 | 2.0 | 5.0 | NA |
| 15-19 | 0.5 | 1.0 | 3.0 | 5.0 | 10 | NA |
| 20-45 | 1.0 | 1.0 | 4.0 | 10 | 35 | NA |
| 46-60 | 1.0 | 1.0 | 4.0 | 10 | 35 | NA |
| 61-69 | NA | NA | 3.0 | 10 | 25 | NA |
| 70-80 | NA | NA | 3.0 | 10 | 25 | NA |
| 81-90 | NA | NA | 3.0 | 5.0 | 20 | NA |
| 91-100 | NA | NA | NA | 5.0 | 20 | NA |
| 101-125 | NA | NA | NA | NA | NA | NA |
QuickLC™ Purity
QuickLC purification: Twice the Yield in Half the Time of HPLC; Same Purity—Guaranteed!
QuickLC purification is a proprietary purification process that offers purity levels
of 85% and above*—equal
to or greater than the levels obtained by HPLC purification. Extensive testing in
end user laboratories has proven that oligos purified with the QuickLC method perform
identically to oligos that go through the older HPLC process. If you believe that
your QuickLC purity oligos are not performing as good or better than HPLC-purified
oligos, call us and we’ll make it right.
The QuickLC process removes most N-1 and other truncated failure sequences created
during synthesis and achieves purity levels of 85% and above*. Selection of QuickLC purification
ensures delivery of your oligo at HPLC-grade purity; typically within 2 days!
QuickLC is highly recommended for oligos needing HPLC-grade purity or when higher
than typical HPLC yields are required for your experiment.
Guaranteed minimum yields
(OD) for QuickLC purity standard oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | NA | NA | 1.0 | 2.0 | 4.0 | NA |
| 15-19 | NA | NA | 2.0 | 4.0 | 20 | NA |
| 20-45 | NA | NA | 2.0 | 4.0 | 30 | NA |
| 46-60 | NA | NA | 2.0 | 4.0 | 30 | NA |
| 61-69 | NA | NA | 2.0 | 4.0 | 20 | NA |
| 70-80 | NA | NA | 2.0 | 4.0 | 20 | NA |
| 81-90 | NA | NA | 2.0 | 4.0 | 20 | NA |
| 91-100 | NA | NA | NA | 4.0 | 20 | NA |
| 101-125 | NA | NA | NA | NA | NA | NA |
Yields are also guaranteed for QuickLC purified oligos containing the following
modifications with no additional modification:
- IUB Wobbles (degenerate nucleotide sites)
- 5’ 6-FAM [6-FAM]
- 5' Amino C6 Linker [Amino C6]
- 5' Phosphate [Phos]
- 5' Biotin-TEG [BioTEG]
Guaranteed minimum yields
(OD) for QuickLC purity modified oligos (as listed above)
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | NA | NA | 0.5 | 1.0 | 2.0 | NA |
| 15-19 | NA | NA | 1.0 | 2.0 | 10 | NA |
| 20-45 | NA | NA | 1.0 | 2.0 | 30 | NA |
| 46-60 | NA | NA | 1.0 | 2.0 | 30 | NA |
| 61-69 | NA | NA | 1.0 | 2.0 | 20 | NA |
| 70-80 | NA | NA | 1.0 | 2.0 | 20 | NA |
| 81-90 | NA | NA | 1.0 | 2.0 | 20 | NA |
| 91-100 | NA | NA | NA | 2.0 | 20 | NA |
| 101-125 | NA | NA | NA | NA | NA | NA |
HPLC Purity
Standard High Performance Liquid Chromatography (HPLC) purification is recommended
for oligos that require additional purification, such as dual-labeled probes, Molecular
Beacons, and most modified oligos that cannot be purified by our QuickLC purification
method.
HPLC purification removes most N-1 and other truncated failure sequences created
during synthesis and achieves purity levels of 85% and above*. Oligos purified by HPLC typically
ship within 5 days. When faster delivery times are critical, Eurofins MWG Operon
encourages you to consider the QuickLC purification, where it is offered for you
oligo type.
Our oligo purification experts will determine and employ the optimum HPLC technique
for your specific oligos and select only the fractions containing the highest purity
for delivery to your lab. The techniques employed include (1) reversed-phase HPLC
purification that separates oligos on the basis of hydrophobicity; (2) ion exchange
HPLC that separates oligos on the basis of charge.
Guaranteed minimum yields
(OD) for HPLC purity standard oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | 0.5 | 0.5 | 2.0 | 10 |
| 10-14 | NA | NA | 0.5 | 1.0 | 2.0 | 15 |
| 15-19 | NA | NA | 1.0 | 2.0 | 10 | 75 |
| 20-45 | NA | NA | 1.0 | 2.0 | 15 | 100 |
| 46-60 | NA | NA | 1.0 | 2.0 | 15 | 100 |
| 61-69 | NA | NA | 1.0 | 2.0 | 10 | 75 |
| 70-80 | NA | NA | 1.0 | 2.0 | 10 | 75 |
| 81-90 | NA | NA | 1.0 | 2.0 | 10 | 75 |
| 91-100 | NA | NA | NA | 2.0 | 10 | 75 |
| 101-125 | NA | NA | NA | NA | NA | NA |
HPLC purification is also available for oligos with all modifications listed on
our web site, except for cholesterol modifications. Yields are guaranteed for HPLC-purified
oligos containing the following single modifications (no multiple or additional
modifications)
- IUB Wobbles (degenerate nucleotide sites)
- 5’ Amino C6 Linker[Amino C6]
- 5’ Phosphate [Phos]
- 5’ Biotin-TEG [BioTEG] * +
- 5’ 6-FAM [6-FAM] *
- 5’ HEX [5HEX] *
- 5’ TET [5TET] *
- 5’ Cy5 [Cy5] *
- 5’ Cy3 [Cy3] *
* 90 base maximum length
+ Not available in the 50 nmol scale.
Guaranteed minimum yields
(OD) for HPLC purity modified oligos (as listed above)
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | 0.5 | 0.5 | 1.0 | 5.0 |
| 10-14 | NA | NA | 0.5 | 0.5 | 1.0 | 10 |
| 15-19 | NA | NA | 0.5 | 1.0 | 5.0 | 10 |
| 20-45 | NA | NA | 0.5 | 1.0 | 10 | 20 |
| 46-60 | NA | NA | 0.5 | 1.0 | 10 | 20 |
| 61-69 | NA | NA | 0.5 | 0.5 | 2.0 | 10 |
| 70-80 | NA | NA | 0.5 | 0.5 | 2.0 | 10 |
| 81-90 | NA | NA | 0.5 | 0.5 | 1.0 | 5.0 |
| 91-100 | NA | NA | NA | 0.5 | 1.0 | 5.0 |
| 101-125 | NA | NA | NA | NA | NA | NA |
Yields are also guaranteed for the following HPLC purity dual-labeled probes and
Molecular Beacons used in qPCR applications.
|
Description
|
Designator
|
|
3' BHQ 1 + 5' HEX
|
BHQ1a-5HEX
|
|
3' BHQ 1 + 5' TET
|
BHQ1a-5TET
|
|
3' BHQ 1 + 5' 6-FAM
|
BHQ1a-6FAM
|
|
3' BHQ 1 + 5' JOE ^
|
BHQ1a-JOE
|
|
3' BHQ 2 + 5' HEX
|
BHQ2a-5HEX
|
|
3' BHQ 2 + 5' Cy3
|
BHQ2a-Cy3
|
|
3' BHQ 2 + 5' Cy5
|
BHQ2a-Cy5
|
|
3' BHQ 2 + 5' ROX ^
|
BHQ2a-ROX
|
|
3' BHQ 2 + 5' TAMRA
|
BHQ2a-TAMRA
|
|
3' BHQ 2 + 5' Texas Red ^
|
BHQ2a-TxRed
|
|
3' TAMRA + 5' 6-FAM
|
TAMRA-6-FAM
|
|
3' TAMRA + 5' TET
|
TAMRA-5TET
|
|
3' TAMRA + 5' HEX
|
TAMRA-5HEX
|
|
Mol. Beacon, 3' DABCYL + 5' 6-FAM
|
MB-6-FAM
|
|
Mol. Beacon, 3' DABCYL + 5' TET
|
MB-5TET
|
|
Mol. Beacon, 3' DABCYL + 5' HEX
|
MB-5HEX
|
|
Mol. Beacon, 3' DABCYL + 5' Cy5
|
MB-Cy5
|
|
Mol. Beacon, 3' DABCYL + 5' Cy3
|
MB-Cy3
|
|
Mol. Beacon, 3' DABCYL + 5' TAMRA
|
MB-TAMRA
|
|
Mol. Beacon, 3' DABCYL + 5' ROX
|
MB-ROX
|
|
Mol. Beacon, 3' DABCYL + 5' Texas Red
|
MB-TxRed
|
^ Available in 200 nmol and 1 umol scales only.
Guaranteed minimum yields
for HPLC purity dual-labeled qPCR Probes (as listed above)
|
Oligo length
|
10 nmol
|
50 nmol
|
200 nmol / 0.2 µmol
|
1 µmol
|
10 µmol
|
|
15-40
|
NA
|
1 OD
|
2 OD
|
5 OD
|
NA
|
|
15-40
|
NA
|
4 nmol
|
8 nmol
|
20 nmol
|
NA
|
2-Step Purity – Reversed Phase/Ion Exchange
This 2-step purification process uses reversed-phase chromatography followed by
anion exchange purification, allowing a two-dimensional purification based on both
hydrophobicity and charge. This purity level is highly recommended for all oligos
70 bases or longer.
Guaranteed minimum yields
(OD) for Two-Step HPLC purity standard oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | 0.5 | 1.0 | 10 |
| 10-14 | NA | NA | NA | 0.5 | 1.0 | 10 |
| 15-19 | NA | NA | NA | 1.0 | 5.0 | 15 |
| 20-45 | NA | NA | NA | 1.0 | 5.0 | 15 |
| 46-60 | NA | NA | NA | 1.0 | 5.0 | 15 |
| 61-69 | NA | NA | NA | 1.0 | 5.0 | 15 |
| 70-80 | NA | NA | NA | 1.0 | 5.0 | 15 |
| 81-90 | NA | NA | NA | 0.5 | 2.0 | 10 |
| 91-100 | NA | NA | NA | 0.5 | 2.0 | 10 |
| 101-125 | NA | NA | NA | 0.5 | 2.0 | NA |
PAGE Purity
Polyacrylamide gel electrophoresis (PAGE) purification is recommended for oligos
that require the highest level of purification, and achieves purity levels for unmodified
oligos of 90% and above*. Although PAGE purification achieves
the highest purity levels available, recovery of the oligo from the matrix is an
inefficient process and results in lower final yields. Thus PAGE purification is
not recommended as a routine technique. PAGE purification also cannot be used for
oligos shorter than 20 bases.
PAGE separates oligos on the basis of molecular weight and is performed on a denaturing
polyacrylamide gel prepared with stock solutions of acrylamide, urea, and TBE buffer.
The desired oligo band is detected through UV shadowing and the full-length product
is excised and eluted from the gel.
Guaranteed minimum yields
(OD) for PAGE purity standard oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | NA | NA | NA | NA | NA | NA |
| 15-19 | NA | NA | NA | NA | NA | NA |
| 20-45 | NA | NA | 1.0 | 2.0 | 10 | NA |
| 46-60 | NA | NA | 0.5 | 2.0 | 4.0 | NA |
| 61-69 | NA | NA | 0.5 | 2.0 | 4.0 | NA |
| 70-80 | NA | NA | 0.5 | 1.0 | 4.0 | NA |
| 81-90 | NA | NA | 0.5 | 1.0 | 2.0 | NA |
| 91-100 | NA | NA | NA | 0.5 | 2.0 | NA |
| 101-125 | NA | NA | NA | 0.5 | 1.0 | NA |
PAGE purification is available for oligos with all modifications listed on our web
site, except for wobbles. Yields are also guaranteed for PAGE-purity oligos containing
the following single modifications (no multiple or additional modifications)
- 5’ Amino C6 Linker [Amino C6]
- 5’ Phosphate [Phos]
- 5’ Biotin-TEG [BioTEG] *
- 5’ 6-Fam [6-FAM]
- 5’ HEX [5HEX] *
- 5’ TET [5TET] *
- 5’ Cy5 [Cy5] *
- 5’ Cy3 [Cy3] *
* 90 base maximum length
Guaranteed minimum yields
(OD) for PAGE purity modified oligos (as listed above)
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | NA | NA | NA | NA | NA | NA |
| 15-19 | NA | NA | NA | NA | NA | NA |
| 20-45 | NA | NA | 0.5 | 1.0 | 5.0 | NA |
| 46-60 | NA | NA | 0.5 | 1.0 | 5.0 | NA |
| 61-69 | NA | NA | 0.5 | 1.0 | 5.0 | NA |
| 70-80 | NA | NA | 0.5 | 1.0 | 5.0 | NA |
| 81-90 | NA | NA | 0.5 | 0.5 | 1.0 | NA |
| 91-100 | NA | NA | NA | 0.5 | 1.0 | NA |
| 101-125 | NA | NA | NA | 0.5 | 0.5 | NA |
2-Step Purity - Reversed Phase/PAGE
This 2-step purification process uses reversed-phase (RP) chromatography followed
by PAGE, allowing a two-dimensional purification based on hydrophobicity and molecular
weight. This purification level is highly recommended for oligos 70 bases or longer
that are used in applications that require PAGE purification. 2-step RP/PAGE purification
is also recommended for oligos used in applications that require the highest PAGE
purity, such as crystallography and gel-shift assays.
Guaranteed minimum yields
(OD) for 2-Step PAGE purity standard oligos
| Oligo length | 10 nmole | 25 nmole | 50 nmole | 0.2 µmole | 1.0 µmole | 10 µmole |
| 6-9 | NA | NA | NA | NA | NA | NA |
| 10-14 | NA | NA | NA | NA | NA | NA |
| 15-19 | NA | NA | NA | NA | NA | NA |
| 20-45 | NA | NA | NA | 1.0 | 2.0 | NA |
| 46-60 | NA | NA | NA | 1.0 | 2.0 | NA |
| 61-69 | NA | NA | NA | 1.0 | 2.0 | NA |
| 70-80 | NA | NA | NA | 0.5 | 1.0 | NA |
| 81-90 | NA | NA | NA | 0.5 | 0.5 | NA |
| 91-100 | NA | NA | NA | 0.5 | 0.5 | NA |
| 101-125 | NA | NA | NA | 0.5 | 0.5 | NA |
*We cannot guarantee the purity of oligos
longer than 70 bases. For oligos longer than 70 bases, Eurofins MWG Operon strongly
recommends one of our 2-Step Purity options.